abstract：:Five criteria for poor response to a 20 min venous occlusion test were applied to 58 patients 3 months or more after acute deep vein thrombosis (DVT). The criteria were arbitrarily defined as the last 5 percentiles of response distributions in an age- and sex-matched healthy control group of 51 subjects. The criteria ...

journal_title：Thrombosis research

authors： Stegnar M,Peternel P,Keber D,Vene N

更新日期：1991-11-15 00:00:00

abstract：:The effects of extracellular sodium on platelet aggregation and calcium mobilization in platelets stimulated with either rattlesnake (Crotalus atrox) lectin (RSL) or alpha-thrombin were compared. The absence of extracellular sodium had no effect on platelet aggregation or calcium mobilization in response to all levels...

journal_title：Thrombosis research

authors： Wilson-Byl JA,Dockter ME,Gartner TK

更新日期：1991-11-01 00:00:00

abstract：:Lipoprotein-associated coagulation inhibitor (LACI) is a plasma-derived protein that inhibits the tissue factor/factor VIIa/calcium/phospholipid complex in a factor Xa-dependent manner. Recombinant LACI (rLACI) added exogenously to plasma prolonged the activated partial thromboplastin time (APTT) and prothrombin time ...

journal_title：Thrombosis research

authors： Welsch DJ,Novotny WF,Wun TC

更新日期：1991-10-15 00:00:00

abstract：:When single-chain pro-UK is activated by plasmin or kallikrein, the Lys158-Ile159 bond is cleaved, leaving a C-terminal lysine on the A-chain (Lys-UK). Two-chain, high molecular weight urokinase (UK) purified from urine, however, has been shown to contain a phenylalanine residue as the C-terminal of the A-chain (Phe-U...

journal_title：Thrombosis research

authors： Lenich C,Pannell R,Gurewich V

更新日期：1991-10-01 00:00:00

abstract：:The activity of tissue plasminogen activator-plasminogen activator inhibitor-1 (t-PA-PAI-1) complex to convert plasminogen to plasmin was examined by using fibrin autography and casein zymography where plasminogen was added or removed for casein layer. Fibrin autography was more sensitive to the activity of t-PA even ...

journal_title：Thrombosis research

authors： Takada Y,Takada A

更新日期：1991-07-01 00:00:00

abstract：:An abnormal prothrombin has been detected in a 26-year-old female, who had no history of excessive bleeding. Prothrombin activity was approximately 10% when measured using either the classical one-stage assay or the assay with Echis carinatus venom, whereas prothrombin antigen level was normal. In keeping with current...

journal_title：Thrombosis research

authors： Morishita E,Saito M,Asakura H,Jokaji H,Uotani C,Kumabashiri I,Yamazaki M,Hachiya H,Okamura M,Matsuda T

更新日期：1991-06-15 00:00:00

abstract：:The thrombin inhibitory role of antithrombin III (ATIII) and heparin cofactor II (HCII) was studied in vitro using intact and injured rabbit aortae. When intact vessels were loaded with thrombin and then exposed to either heat defibrinogenated human plasma (HDHP) or ATIII the same degree of thrombin inhibition was ach...

journal_title：Thrombosis research

authors： Pasche B,Swedenborg J,Frebelius S,Olsson P

更新日期：1991-06-01 00:00:00

abstract：:We examined the effects of polysaccharides on t-PA mediated plasminogen activation using single-chain tissue plasminogen activator (sct-PA) and two-chain tissue plasminogen activator (tct-PA). Unfractionated heparin, low molecular weight heparin (LMW heparin) and dextran sulfates enhanced the activation rate of plasmi...

journal_title：Thrombosis research

authors： Kawamura H,Watanabe I,Urano T,Takada Y,Takada A

更新日期：1991-06-01 00:00:00

abstract：:The effects of desmopressin and dextran on haemostasis and fibrinolysis were studied in four healthy volunteers. Both drugs were compared to placebo, each volunteer being subject to four experiments. Dextran 70 (30 g i.v.) moderately decreased VIII:C and vWF:Ag and slightly increased antithrombin III, also when haemod...

journal_title：Thrombosis research

authors： Flordal PA,Svensson J,Ljungström KG

更新日期：1991-06-01 00:00:00

abstract：:A simple and reproducible method to induce an occlusive thrombus in rabbit carotid artery is reported. Rabbits were anesthetized and prepared to record arterial pressure, heart rate, and carotid blood flow. A critical stenosis of a damaged carotid artery was obtained using an external plastic cylinder. Complete occlus...

journal_title：Thrombosis research

authors： Bevilacqua C,Finesso M,Prosdocimi M

更新日期：1991-05-15 00:00:00

abstract：:All the thrombolytic agents currently in clinical use act as plasminogen activators. In this study evidence is presented that also oxidants of the phagocyte type are of fibrinolytic efficiency in vivo. Activated phagocytes participate in physiologic fibrinolysis. The cells generate plasminogen activators and reactive ...

journal_title：Thrombosis research

authors： Stief TW

更新日期：1991-05-01 00:00:00

abstract：:Megakaryocyte colony formation was increased in 13 patients with ITP. Out of splenectomized ITP patients Colony Forming Unit-Megakaryocyte (CFU-Mk) number normalized in four but was lower than normal in the remaining two. The proportion of immature megakaryocytes was higher in ITP than in normals or in ITP patients af...

journal_title：Thrombosis research

authors： Podolak-Dawidziak M

更新日期：1991-04-01 00:00:00

abstract：:The susceptibility of von Willebrand factor /vWF/ to digestion by human neutrophil cathepsin G in highly purified FVIII/vWF concentrate /BHS/ and in cryoprecipitate has been studied. In contrast to human neutrophil elastase, cathepsin G inactivates and degrades vWF not only in BHS but also in cryoprecipitate. Fibrinog...

journal_title：Thrombosis research

authors： Jeleńska M,Kopeć M,Sopata I,Vigh Z,Scharrer I,Breddin K

更新日期：1991-02-15 00:00:00

abstract：:We previously reported that the expression of thrombomodulin on the MEG-01, a cell line from human megakaryoblastic leukemia, was increased by agents that increase intracellular cAMP. In this paper we examine the effect of these agents on cultured human umbilical vein endothelial cells (HUVEC) and mouse hemangioma cel...

journal_title：Thrombosis research

authors： Maruyama I,Soejima Y,Osame M,Ito T,Ogawa K,Yamamoto S,Dittman WA,Saito H

更新日期：1991-02-01 00:00:00

abstract：:The immunogenic potential of natural and recombinant hirudin was investigated using baboons. Animals received 4 intravenous hirudin injections of 1,000 antithrombin units/kg at certain times (day 1, 3, 8 and 42) and were checked for an immune response. None of the tests performed (in vitro histamine-release, ELISA for...

journal_title：Thrombosis research

authors： Bichler J,Gemmerli R,Fritz H

更新日期：1991-01-01 00:00:00

abstract：:Whole blood electrical aggregometry (WBEA) has become an accepted method to gain quick information on platelet disorders. Compared to the optical method WBEA is closer to physiology and less complicated, but on the other hand more difficult to standardize. Different approaches have been attempted in the past to improv...

journal_title：Thrombosis research

authors： Müller MR,Schreiner W,Wohlfahrt A,Salat A,Wolner E

更新日期：1990-12-15 00:00:00

abstract：:Severe prekallikrein (Fletcher factor) deficiency was diagnosed in a 49-year-old woman and in 3 of her siblings. Functional prekallikrein (PKK) activity was found below 1% by clotting assay and 20% by amidolytic assay in all the affected subjects; PKK cross-reacting material (CRM) was present in all the patients (anti...

journal_title：Thrombosis research

authors： De Stefano V,Leone G,Teofili L,De Marinis L,Micalizzi P,Fiumara C,Bizzi B

更新日期：1990-12-01 00:00:00

abstract：:Platelet aggregation in platelet-rich plasma (PRP) is more sensitive to agonists and more resistant to antagonists than that in whole blood. In this study, we show that the presence of neutrophils in whole blood accounts at least in part for diminished platelet aggregation in whole blood. The platelet-inhibitory effec...

journal_title：Thrombosis research

authors： Nicolini FA,Mehta JL

更新日期：1990-12-01 00:00:00

abstract：:SK, t-PA or APSAC were incubated in human plasma (adjusted to 300,000 platelets/mm3), in vitro, for up to 90 minutes using concentrations which were equivalent to those achieved in the treatment of AMI patients. Aggregation was measured in response to ADP and collagen. SK inhibited platelet aggregation after a 60 minu...

journal_title：Thrombosis research

authors： Fears R,Ferres H,Greenwood HC

更新日期：1990-11-15 00:00:00

abstract：:Factor IX Nagoya 3 (IX Nagoya 3) is a natural mutant of factor IX recognized in a patient with moderately severe hemophilia B. The patient had 0.60 units/ml of factor IX antigen and 2-5% of clotting activity. IX Nagoya 3 was purified from the patient's plasma by immunoaffinity chromatography with an anti-factor IX mon...

journal_title：Thrombosis research

authors： Suehiro K,Miyata T,Takeya H,Takamatsu J,Saito H,Murakawa M,Okamura T,Niho Y,Iwanaga S

更新日期：1990-11-15 00:00:00

abstract：:Incubation of recombinant human tissue factor apoprotein (Apo-TF) with human plasma decreased the recalcified clotting time of this plasma in a time-and dose-dependent manner suggesting relipidation of the Apo-TF by plasma lipoproteins. Incubation of Apo-TF with purified preparations of human very low density, low den...

journal_title：Thrombosis research

authors： Sakai T,Kisiel W

更新日期：1990-11-01 00:00:00

abstract：:The anticoagulant properties of myxalin, a glycopeptide secreted by a Gram negative bacterium strain (Myxococcus xanthus) are studied and compared to those of heparin. This soluble material exhibits an anticoagulant activity which implies the inhibition of some serine proteases, thrombin and factor Xa. In the presence...

journal_title：Thrombosis research

authors： Akoum A,Josefonvicz J,Sigot M

更新日期：1990-10-01 00:00:00

abstract：:Four murine monoclonal antibodies against rat platelets were produced by fusion of spleen cells from mice intravenously immunized with whole rat platelets. All four antibodies immunoprecipitated two major platelet membrane proteins with apparent molecular weights of 130,000 and 82,000 (nonreduced) and of 120,000 and 9...

journal_title：Thrombosis research

authors： Miyazaki H,Tamura S,Sudo T,Suzuki T

更新日期：1990-09-15 00:00:00

abstract：:Blood was obtained from four patients envenomated by the Australian common brown snake, Pseudonaja textilis textilis. This elapid snake has one of the most toxic venoms in the world, containing extremely potent neurotoxic and coagulant components. The latter is a potent complete prothrombinase, converting prothrombin ...

journal_title：Thrombosis research

authors： Masci PP,Rowe EA,Whitaker AN,de Jersey J

更新日期：1990-09-01 00:00:00

abstract：:Thrombin was acetylated by treatment with acetic anhydride, and the potency for protein C activation and the fibrinogen clotting activity of the resultant acetylthrombin were investigated in vitro and in vivo. The acetylthrombin retained an amidolytic activity to the synthetic substrate, S-2238, and reduced both of th...

journal_title：Thrombosis research

authors： Nakagomi K,Ajisaka K,Yokota I

更新日期：1990-08-15 00:00:00

abstract：:To compare Canola a terrestrial (n-3) oil with fish oil (n-3) and common vegetable oil (n-6) in decreasing serum lipids and platelet aggregation, various oils were included in Chow-based diets and fed to rats in an eight-weeks feeding trial. The Chow diet contained 2% cholesterol along with either Canola, menhaden, sa...

journal_title：Thrombosis research

authors： Watkins T,Lenz P,Bierenbaum M

更新日期：1990-08-01 00:00:00

abstract：:Human tissue plasminogen activator (t-PA) was shown to bind specifically to human osteosarcoma cells (HOS), and human epidermoid carcinoma cells (A-431 cells). Crosslinking studies with DTSSP demonstrated high molecular weight complexes (130,000) between 125I-t-PA and cell membrane protein on human umbilical vein endo...

journal_title：Thrombosis research

authors： Beebe DP,Wood LL,Moos M

更新日期：1990-07-15 00:00:00

abstract：:Tissue factor, the cofactor for factor VIIa-catalyzed activation of factors IX and X, plays an important role in the initiation of hemostasis. However, the distribution of tissue factor in the body has not been defined until recently. In the present study frozen sections of non-malignant human tissues were immunostain...

journal_title：Thrombosis research

authors： Fleck RA,Rao LV,Rapaport SI,Varki N

更新日期：1990-07-15 00:00:00

abstract：:Activation of plasma plasminogen to plasmin is the objective of current strategies for thrombolytic therapy. Although thrombolytic activity in blood involves a large cellular component as well as that in plasma, the contribution of this cellular phase to clinical thrombolysis has not been examined. Using a 125I-fibrin...

journal_title：Thrombosis research

authors： Langleben D,Moroz LA,Schlesinger RD

更新日期：1990-07-15 00:00:00

abstract：:The study of the interaction between activated protein C (APC) and non-plasmatic inhibitors allowed us to demonstrate that aprotinin is a potent competitive inhibitor of APC with a Ki of 1.35 mumol/L. It was possible to adsorb immunopurified protein C (PC) activated by venom activator to insolubilized aprotinin and to...

journal_title：Thrombosis research

authors： Taby O,Chabbat J,Steinbuch M

更新日期：1990-07-01 00:00:00

abstract：:Four monoclonal antibodies have been produced, which are specific for the Ca2+ or Sr2(+)-induced conformation of human factor IX. Certain, but not all, gamma-carboxy-glutamic acid residues in factor IX are involved in the epitope expression together with the conformation stabilized by the adjacent region of Gla-domain...

journal_title：Thrombosis research

authors： Sugo T,Mizuguchi J,Kamikubo Y,Matsuda M

更新日期：1990-06-15 00:00:00

abstract：:The concentrations of tissue plasminogen activator (t-PA) and urokinase (UK) were measured in the plasma of patients with benign or malignant ovarian and uterine tumors. Plasma levels of t-PA were higher in patients with malignant ovarian and uterine tumors than in patients with benign tumors. Plasma UK levels were, h...

journal_title：Thrombosis research

authors： Saito K,Nagashima M,Iwata M,Hamada H,Sumiyoshi K,Takada Y,Takada A

更新日期：1990-05-15 00:00:00

abstract：:A platelet storage pool deficiency (SPD) is present in platelets from cattle with the Chediak-Higashi syndrome (CHS). The most plausible hypothesis for the SPD is that dense granule precursors are simply not formed in CHS megakaryocytes. There is, however, evidence that some recently acquired 5-hydroxytryptamine (5HT)...

journal_title：Thrombosis research

authors： Meyers KM,Costa JL,Magnuson J

更新日期：1990-05-01 00:00:00

abstract：:Severe bleeding took place in a patient when abdominal aneurysm was removed by operation. Bleeding continued after infusion of heparin, antithrombin III (ATIII), fresh platelets and fresh blood. Infusion of tranexamic acid resulted in an immediate cessation of bleeding and improvement of his general condition. Sometim...

journal_title：Thrombosis research

authors： Takada A,Takada Y,Mori T,Sakaguchi S

更新日期：1990-04-15 00:00:00

abstract：:This study investigates the effect on human platelet function of two sesquiterpene lactones from Arnica montana L., helenalin (H) and 11 alpha,13-dihydrohelenalin (DH). Both compounds inhibited collagen-induced platelet aggregation, thromboxane formation and 5-hydroxytryptamine secretion in a concentration-dependent m...

journal_title：Thrombosis research

authors： Schröder H,Lösche W,Strobach H,Leven W,Willuhn G,Till U,Schrör K

更新日期：1990-03-15 00:00:00

abstract：:Endothelial cells from human umbilical vein were incubated in vitro with thrombin in the absence or presence of unfractionated heparin (UFH), low molecular weight heparin (LMWH) or dermatan sulfate (DS) to clarify the effect of these glycosaminoglycans on thrombin-stimulated prostaglandin I2 (PGI2) production. Althoug...

journal_title：Thrombosis research

authors： Itoh F,Kaji T,Hayakawa Y,Oguma Y,Sakuragawa N

更新日期：1990-02-15 00:00:00

abstract：:The ability of the COA-SET Fibrin monomer (COA-SET FM) test to detect soluble fibrin was evaluated by comparing the results of the COA-SET FM test with fibrinopeptide A (FPA) determinations following thrombin incubation of plasma or whole blood. In addition, two semiquantitative tests (erythrocytes-agglutination test ...

journal_title：Thrombosis research

authors： Halvorsen S,Skjønsberg OH,Ruyter R,Godal HC

更新日期：1990-02-15 00:00:00

abstract：:Protein C (PC) is the central protein in a major antithrombotic regulatory mechanism. Hereditary deficiencies of PC are associated with thrombosis. Therapeutic PC replacement may be an important treatment if pure functional human protein C is available in sufficient quantity. Human PC has been produced on a commercial...

journal_title：Thrombosis research

authors： Madden RM,Oppenheimer C,Wydro R,Marlar RA

更新日期：1990-02-01 00:00:00

abstract：:Lupus anticoagulants (LA) are IgG or IgM antibodies which prolong phospholipid-dependent coagulation tests. For the detection and quantitation of such antibodies, we have developed an ELISA with cephalin as the coating antigen. The sensitivity of this assay was compared to the activated partial thromboplastin time (AP...

journal_title：Thrombosis research

authors： Sletnes KE,Keirung G,Wisløff F

更新日期：1990-01-15 00:00:00

abstract：:A Ca++-ionophore, ionomycin, increased the volume of human platelets suspended in a Ca++-containing buffer. This change in cell volume was dependent upon ionomycin and extracellular Ca++ concentrations, suggesting that the volume change occurs when the intracellular Ca++ reaches a certain level (greater than uM as det...

journal_title：Thrombosis research

authors： Ozaki Y,Matsumoto Y,Yatomi Y,Higashihara M,Kariya T,Kume S

更新日期：1990-01-01 00:00:00